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The Fascinating World of Bacteria

Bacteria growing in a petri dish

In This Issue:

  • Bacteria: Tiny But Tough
  • Stop the Bacteria: Soap Survey Project
  • Science Links

 

Bacteria may be microscopic in size, but they make up for it in sheer number. You have millions of bacteria living on your skin alone, and lots more inside of you! Does that thought give you the creeps? We usually think of bacteria as the harmful germs that make us sick, and while that is often true, there are lots of other things bacteria do that we couldn't live without. Read on for some fascinating facts about bacteria: where they live, how they can harm us, and how they can help us.

Where They Live

Where they live is one of the most surprising things about bacteria. The truth is, they live everywhere, even places on earth where we once thought nothing could survive!

Bacteria coloring the water in a hot spring

In the heat. The hot springs of Yellowstone National Park contain highly toxic, sometimes boiling water. In this environment, where most living things couldn't survive, bacteria called thermophiles (heat-lovers) thrive. These bacteria are fueled by hydrogen and sulfur in the water, and they produce many of the brilliant colors in Yellowstone's hot springs.

Other thermophiles live near hydrothermal vents on the ocean floor. These vents are usually more than 7,000 ft (1.3 miles) below the surface, and at this depth the water pressure is extreme—it would crush us in no time at all! In addition, the temperature fluctuates drastically between hot vent water pouring out from cracks in the ocean floor and cold sea water. There is no natural light that deep in the sea, and the water spouting from the vents is full of harsh chemicals and minerals. And yet bacteria live there, and even help other creatures (like tubeworms) live by converting the toxic chemicals from the vents into food for them.

In the cold. Most bacteria don't grow as well in colder temperatures, which is why putting your food in the refrigerator helps keep it from spoiling. There are bacteria called psychrophiles (cold-lovers), though, that can live and reproduce in cold temperatures €”even below the freezing point. Researchers in Antarctica have discovered bacteria in ice samples taken from about 11,700 feet deep in the ice above Lake Vostok.

In radioactive environments. One type of bacteria, called Dienococcus radiodurans, can survive lethal amounts of radiation, up to 1000 times more than would kill a human!

Inside you. Bacteria live on your skin, on your teeth, on your tongue, in your intestines, in your eyes, and more. You are host to millions of them! Some of them can harm you, but many of them help you. How? Keep reading to find out! But first: what exactly are bacteria?

What They Are

Bacteria are one-celled or unicellular microorganisms. They are different from plant and animal cells because they don't have a distinct, membrane-enclosed nucleus containing genetic material. Instead, their DNA floats in a tangle in the interior of the cell. Individual bacteria can only be seen with a microscope, but they reproduce so rapidly that they often form colonies that we can see. Bacteria reproduce when one cell splits into two cells in a process called binary fission. Fission occurs rapidly in as little as 20 minutes. Under perfect conditions a single bacterium could grow into over one billion bacteria in only 10 hours! (It's a good thing natural conditions are rarely perfect, or the earth would be buried in bacteria!)

How They Can Harm Us

Disease-causing bacteria are called pathogenic. These come in many forms and can cause illnesses from an ear infection to strep throat to cholera. They can get into our bodies via our mouth and nose, or through cuts and scrapes. Some are airborne, others are found in food, resulting in food poisoning. Bacteria are also the cause of plaque buildup on our teeth, which can lead to cavities and gum disease.

Before the discovery of antibiotics, many severe bacterial diseases had no cure and usually resulted in death. Antibiotics work by destroying bacteria or inhibiting their reproduction while leaving the body's own cells unharmed. After a time, some bacteria develop resistance to an antibiotic, and it will no longer be effective against them. Because of this, scientists are always researching new antibiotics. (Many diseases, such as chicken pox, hepatitis, or polio, are caused by viruses rather than bacteria. Antibiotics have no effect against these diseases.)

Bacterial infections are common, but many of them can be avoided by good cooking, cleaning, and hand-washing practices.

How They Can Help Us

Where would we be without bacteria? Well, we might not be getting bacterial diseases, but we would still be a lot worse off! Bacteria perform all sorts of very important functions, both in our bodies and in the world around us. Here are just a few.

Digestion. Our large intestines are full of beneficial bacteria that break down food that our bodies can't digest on their own. Once the bacteria break it down, our intestines are able to absorb it, giving us more nutrients from our food.

Vitamins. Bacteria in our intestines actually produce and secrete vitamins that are important for our health! For example, E. coli bacteria in our intestines are a major source of vitamin K. (Most E. coli is good for us, but there is a harmful type that causes food poisoning.)

Food. Bacteria are used to turn milk into yogurt, cheese, and other dairy products.

Oxygen. Cyanobacteria (which used to be called blue-green algae) live in water and perform photosynthesis, which results in the production of much of the oxygen we need to breathe.

Cleanup. Oil spills, sewage, industrial waste €”bacteria can help us clean all of these up! They "eat" the oil or toxins and convert them into less harmful substances.

Bacteria are amazing creatures, aren't they? They can be so dangerous and yet so important at the same time. Since they are everywhere, you can easily experiment with them yourself, even without a microscope. Try it out with the science project below.

Stop the Bacteria: Soap Survey Project

Every time you touch something you are probably picking up new bacteria and leaving some behind. This is how many infectious diseases spread €”we share our bacteria with everyone around us! Even bacteria that lives safely on our skin can make us sick if it gets inside our bodies through our mouths or cuts and scrapes. This is one reason why it is so important that we wash our hands frequently and well.

What kind of soap works best for cutting down on the bacteria on our hands? You can test this by growing some bacteria cultures using agar and petri dishes.

Materials

What To Do

  1. Prepare the agar according to the directions on the label, then pour enough to cover the bottom of each petri dish. Cover the dishes and let them stand for about an hour until the agar has solidified again. (If you aren't going to use them right away after they have cooled, store them upside down in the refrigerator.)
  2. When your petri dishes are ready, collect some bacteria from your hand or the hand of a volunteer. (Make sure the person hasn't washed his or her hands too recently!) Do this by rubbing the sterile swab over the palm in a zigzag pattern.
  3. Remove the cover from the petri dish and lightly rub the swab back and forth in a zigzag pattern on the agar. Turn the dish a quarter turn and zigzag again. Cover the dish and repeat steps two and three for the other dish, using a new sterile swab. Label the dishes "Test" and "Control." (You may want to do more than one test dish, so you can compare the results.)
  4. Cut the blotter paper into small "sensitivity squares." Use permanent ink to label the squares for the different types of hand cleaners you are going to test, e.g., "R" for regular soap, "A" for antibacterial soap, and "S" for hand sanitizer. Using tweezers, dip each square into the appropriate cleaner. Blot the excess cleaner on a paper towel and then place the squares on the agar in the "Test" dish. (Spread the squares out so there is distance between them.) Add one square of plain blotter paper to test if blotter paper by itself has any effect. Don't put any squares in the "Control" dish - this one will show you what the bacterial growth will look like without any soap.
  5. Put the dishes in a dark, room-temperature place like a closet and leave them undisturbed for a few days.
Bacteria growing in the control dish

After 3-7 days, take your petri dishes out and observe the bacteria growth (without removing the lids). There might be some mold growth, too, since you may have swabbed microscopic mold and fungi spores along with the bacteria. Compare the amount of bacteria in the control dish to the amount in the test dish. Next, compare the amount of bacteria growth around each paper square. Which one has bacteria growing closest to it? Which one has the least amount of bacteria growing near it?   If you did more than one test dish, are the results similar in all the test dishes? If not, what variables do you think might have caused the results to be different?

(Remember to use care when experimenting with bacteria. The kind of bacteria you're using in these projects are types that are normally present in your house, but you are culturing them in greater numbers than usual, and this can be hazardous. When you're finished with your experiment, pour a little bleach into each petri dish, seal the dishes in a plastic bag, and throw them away.)

Which soap inhibited bacteria growth the most? Which one would you recommend using when you wash your hands? What other substances could you test for antibacterial effects? Try some of these ideas:

  • Household cleaners. Which household cleaners work best against bacteria? Try swabbing a surface in your home, like the kitchen sink or a toilet, and then use sensitivity squares to test different cleaners such as Lysol, bleach, Windex, etc.
  • Natural substances. Test to see if garlic really has antibacterial properties. What about tea tree oil, or red pepper, or curry?
  • Mouthwash. Swab your teeth and gums and see how well toothpaste or mouthwash work against the plaque-causing bacteria on your teeth.
  • Antibiotics. Use an antibiotic disc set to see what different antibiotics can do against bacteria. For a more advanced project, learn how gram staining relates to the use of antibiotics.

Don't stop there—there are lots of other projects you can do with bacteria

  • Have you heard people say that dogs' mouths are cleaner than humans'? Design an experiment to test whether this is really true!
  • Some band-aids are advertised as being antibacterial. Test to see if they really work better than regular band-aids at inhibiting bacteria.
  • Is it safe to keep refilling a water bottle without washing it? Test a sample of water from the bottom of a water bottle that has been used for a couple days and compare it to a sample from a freshly-opened, clean water bottle. You can also test to see if a bottle gets more bacteria in it if you drink with your mouth or with a straw.
  • Does bacteria grow in your shoes? Is there a difference in bacteria growth between fabric shoes and leather? Do foot powders work to cut down on bacteria?
  • Does bacteria grow on your toothbrush? What are some ways you could try to keep it clean? Mouthwash? Hot water?
  • Some people recommend getting a new mascara every 6 weeks because bacteria can grow in the tubes. Test this by comparing bacteria growth from old mascara and new, unused mascara.
  • What about good bacteria? Try making some homemade yogurt with this kind!

As you experiment, you'll notice that the bacteria growth in your petri dishes will often be different colors or textures. These are colonies of different types of bacteria. If you have a microscope, look at a sample of each colony and observe the differences. Bacteria come in three different shapes; see if you can distinguish these shapes under the microscope.

Science Links

Ready for some candy that will help prevent cavities?   A UCLA microbiologist has invented lollipops that kill bacteria that cause tooth decay!

Bacteria come in all sorts of fascinating shapes and sizes. Check out some great images in the MicrobeWorld photo gallery.

Want to know about the biggest, smallest, and deadliest bacteria? Visit MicrobeWorld's Microbial Book of World Records.

Interested in more articles like this? Sign up for our e-mail newsletters to get new science experiments, cool facts, and more each month!

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